ABSTRACT
Breeding for high and low hypothermic responses to systemic administration of a serotonin1A (5-HT1A) receptor agonist (8-hydroxy-2-(di-n-propylamino)tetralin, 8-OH-DPAT) has resulted in high DPAT-sensitive (HDS) and low DPAT-sensitive (LDS) lines of rats, respectively. These lines also differ in several behavioral measures associated with stress. In the present microdialysis study we observed that basal 5-HT concentrations in the prefrontal cortex and dorsal hippocampus did not differ significantly between HDS and LDS rats. Thus, behavioral differences between the HDS and LDS lines might not be attributed to differences in basal 5-HT release. However, both lines had lower basal levels of 5-HT release than their randomly bred control group (random DPAT-sensitive, RDS) in the prefrontal cortex (mean ± SEM, pg/20 æl, was 3.0 ± 0.4 for LDS, 3.8 ± 0.3 for HDS and 6.4 ± 0.6 for RDS; F(2,59) = 5.8, P<0.005). The administration of (±)-fenfluramine (10 mg/kg) induced a greater increase in hippocampal 5-HT levels in HDS rats (500 percent) as compared with LDS (248 percent) or RDS (243 percent) rats (P<0.0001). There were no significant differences in the prefrontal cortex among lines, with a fenfluramine-induced 5-HT increase of about 900 percent in the three groups. This differential response to fenfluramine may be due to functional alterations of hippocampal 5-HT reuptake sites in the HDS line
Subject(s)
Animals , Rats , 8-Hydroxy-2-(di-n-propylamino)tetralin , Brain , Fenfluramine , Receptors, Serotonin , Serotonin , Serotonin Receptor Agonists , Selective Serotonin Reuptake Inhibitors , Analysis of Variance , Brain , Breeding , Cerebral Cortex , Hippocampus , Hypothermia , Microdialysis , Species SpecificityABSTRACT
Se ha efectuado el procedimiento de microdiálisis cerebral durante talamotomía estereotáxica N.V.L en 3 pacientes de enfermedad de parkinson, para corregirles el síndrome del temblor. Se realizó correlación entre la concentración intersticial del GABA y los cambios de intensidad del tremor. La microdiálisis es un método seguro, reproductible y fácil de practicar durante operaciones neuroquirúrgicas estereotáxicas. Se expone los resultados preliminares. Debido a la muestra de 3 pacientes, no se puede establecer conclusiones definitivas. Esta investigación está actualmente en progreso